| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035432 |
| Chromosome: | chromosome 1 |
| Location: | 2320755 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013000 | (1 of 1) 2.7.7.31 - DNA nucleotidylexotransferase / Terminal transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCCCCAGCTGCAGTAGCCGCACGCAGCGTGTCAGCACACGCCCTGTT |
| Internal bar code: | ATTTACGATGGCTCCCGAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAATGAGGGCCATGGTG |
| Suggested primer 2: | GTCCGCTTACTTGTCGGTCA |