| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035440 |
| Chromosome: | chromosome 12 |
| Location: | 3253706 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499451 | (1 of 1) PTHR14089//PTHR14089:SF2 - CELL CYCLE CONTROL PROTEIN // PRE-MRNA-SPLICING FACTOR CWC2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGATCAAGGTGGTGCGGCGGCGGCAGGGACAGCGGGGACGGAGGG |
| Internal bar code: | TCAATTATTGTGTTGCCTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1969 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTTTGCACGCCGTTAA |
| Suggested primer 2: | CACCAAGGGGAAAAACAGCG |