Insertion junction: CLIP2.035442_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.035442
Chromosome:chromosome 16
Location:1159287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g650350 (1 of 1) PF03457 - Helicase associated domain (HA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCTTCACCGCCCTCCCCCCCCCAACACCAGGACATCTACTCCGCACG
Internal bar code:GAGTTTGGGCATAGTTCGCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTCATGTCCAGCAGTCG
Suggested primer 2:GCTGACATGGCGTATTGCTG