| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035476 |
| Chromosome: | chromosome 16 |
| Location: | 7005206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673281 | (1 of 1) K00477 - phytanoyl-CoA hydroxylase (PHYH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGACTCTACACACATACGGTACAGGAAATTGGACACTACAACCAGCGA |
| Internal bar code: | ACGCTGTGTTAACGTCAAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTGCAAGTGATAAGCC |
| Suggested primer 2: | ATGCTGGGCTGCTTGAAGAT |