| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035509 |
| Chromosome: | chromosome 3 |
| Location: | 7320465 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206850 | (1 of 2) IPR004911//IPR012336 - Gamma interferon inducible lysosomal thiol reductase GILT // Thioredoxin-like fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCTCATGGTGGGGCAGGCGCGTGGAGGTGCTTCTGGCGGTCATGAG |
| Internal bar code: | GGGTGGTACAATAGTTCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1299 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTGCTCAGGCTACGCT |
| Suggested primer 2: | CTGTCGACACGAACCAGACA |