| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035526 |
| Chromosome: | chromosome 10 |
| Location: | 5973681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461350 | BUG23 | Basal-body proteins with upregulated genes 23 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCTCGTACCGCAGCGCCATCAGTGCCTTGGCGGCCTCGTCGCTCTTG |
| Internal bar code: | AACGCATAAATGACTACAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3419 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAGGACGCGGAGGTAGGA |
| Suggested primer 2: | CGGCAAAAGCAAACACAGGA |