| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035526 |
| Chromosome: | chromosome 10 |
| Location: | 5973693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461350 | BUG23 | Basal-body proteins with upregulated genes 23 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGATGCCGTACCTGCGGGAGGGGGCTGGGAGCTGCGGGTTGTTGG |
| Internal bar code: | TTCGTTCGATAGCGGTTTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCAAAAGCAAACACAGGA |
| Suggested primer 2: | TAAAGGACGCGGAGGTAGGA |