| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035535 |
| Chromosome: | chromosome 9 |
| Location: | 4500059 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400886 | (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATCCAAATGATGGAAGCGTAGTGCGTGTGTGGGTCGGTGGCGCTGCT |
| Internal bar code: | CCTAAAAAGAACTACGTGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1572 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAGGAAAACAAAGCGCA |
| Suggested primer 2: | TGGCACCTCCATAAGCTGTG |