Insertion junction: CLIP2.035537_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.035537
Chromosome:chromosome 6
Location:6998658
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g297082 (1 of 2) 2.7.11.1//4.6.1.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Adenylate cyclase / ATP pyrophosphate-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTGGGGGCTGGGGCCAGCTCGCAACTGGCCCAATCGCTGGCGTCGCC
Internal bar code:TTTTGACAAAGAATGGATGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1543
LEAP-Seq percent confirming:31.9149
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGCTGCGTCAGTATGTG
Suggested primer 2:GGCAGGGAATTGGGAGGTAC