Insertion junction: CLIP2.035548_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.035548
Chromosome:chromosome 16
Location:277216
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g694250 (1 of 18) PF12937 - F-box-like (F-box-like) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGAATGTCCTCGCACCCCAAGCCACGCCCCTTGCCCCAGCCACCACA
Internal bar code:TTTTCTGAATTTCCGCATCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1260
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGATAGAGCAATCGCGT
Suggested primer 2:CGCTTTGCTTTCCTTGTGCT