| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035566 |
| Chromosome: | chromosome 16 |
| Location: | 1989835 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656551 | TRL14,TRXL14,PDI | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCAGCAGAAGCGACCGGACCACATACAGGAGCCGGGACGAGTTGTCT |
| Internal bar code: | AGAAAGTAACTAGGGAGAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6597 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCAACCACCGTGGGATC |
| Suggested primer 2: | CCAGGGACGGCTTTGTTTTG |