| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035570 |
| Chromosome: | chromosome 17 |
| Location: | 2069084 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGAGGATCCGAGGCCCTCGGGCGCCGTTTCCCGCCACACTTTGGCGAT |
| Internal bar code: | ACACGTGCGCTCCGATTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1811 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACAAGGAGCTGCATGAC |
| Suggested primer 2: | CCCACAGGTCTACCGTTTCC |