| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.035579 |
| Chromosome: | chromosome 7 |
| Location: | 4380245 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340950 | AXL4 | (1 of 6) PTHR10994//PTHR10994:SF61 - RETICULON // RETICULON-LIKE PROTEIN; Arabinose chain extension enzyme like protein 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGGTCACCGCCAAGGCCGGCCTGACCGCGGCGGCGCACGAGCCCGG |
| Internal bar code: | ACTTTGGATTCAGGAAGACTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 61.9048 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGGTTGGGCGAGATGG |
| Suggested primer 2: | GTTTGAGAACCCGTTGTGCC |