Insertion junction: CLIP2.035602_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.035602
Chromosome:chromosome 11
Location:3456333
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTTGGGGGTGGGACAGCCGACGGGTTTGTTTGCAGTTGCCAATCATCG
Internal bar code:GAAGTCGTCGTGTAAAGAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2443
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACCCCAGAGTATAGCG
Suggested primer 2:CCCCTTCCCCATCTACCAGA