| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035606 |
| Chromosome: | chromosome 7 |
| Location: | 497680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800769 | (1 of 2) PF01124 - MAPEG family (MAPEG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGGCGGCACCTTGGGGGTGAGCGGACGGGTGCACGCGCAACTTGTC |
| Internal bar code: | GATCGGCCCTCCCCGTACAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4508 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCCCTCATTGTCATTCC |
| Suggested primer 2: | CTGTTGCGGTGTTGTGACTG |