| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035626 |
| Chromosome: | chromosome 17 |
| Location: | 3481985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723950 | FAP397 | Flagellar Associated Protein 397 with UDP-galactose 4-epimerase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACATAGAAAGTTGGACCGAACCTGGGATGGGATGAGGGTGTTGGCGAT |
| Internal bar code: | GTGAATCGAGAAGTCACCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3319 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAAGGTGCGTTGGGAAC |
| Suggested primer 2: | TTGACTTGTCGTCCAGCTCC |