Insertion junction: CLIP2.035629_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035629
Chromosome:chromosome 1
Location:5609878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040000 RPL26 (1 of 1) K02898 - large subunit ribosomal protein L26e (RP-L26e, RPL26); Cytosolic 80S ribosomal protein L26 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCAATGCCCACGTTCACGACGGCACCTGTGAAGCAGAGGACAACGCAC
Internal bar code:TTAGTGTTACGGTCCCAGCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3700
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGACAGTATAAGCGGCGACT
Suggested primer 2:GAACTGTGCAGACGGAAGGA