| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035649 |
| Chromosome: | chromosome 12 |
| Location: | 1876349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512100 | PIF1 | (1 of 1) IPR003593//IPR003840//IPR010285//IPR013785//IPR027417 - AAA+ ATPase domain // DNA helicase // DNA helicase Pif1-like // Aldolase-type TIM barrel // P-loop containing nucleoside triphosphate hydrolase; {"PIF1-related DNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCACGATGTTCCGGCGCGCTCGGTGGCAGAGGCAGGCCTGGAGAAC |
| Internal bar code: | TCGGGGGACGCCTGCACTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2854 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGGCGAACAATGCAGTA |
| Suggested primer 2: | TCTCCATGATGACGGATGCG |