| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035654 |
| Chromosome: | chromosome 2 |
| Location: | 4481979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106550 | (1 of 1) K12839 - survival of motor neuron-related-splicing factor 30 (SMNDC1, SPF30) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGAGGAGGCGGATGAGGAGCAGTGAGCGAGTAGTGGGCAGGAGCCAG |
| Internal bar code: | TACGACGTATGAAGAGGGGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3769 |
| LEAP-Seq percent confirming: | 92.1569 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAACCTGCGTGGCATATG |
| Suggested primer 2: | AGCAAGATCCGCTTCCAACA |