| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035674 |
| Chromosome: | chromosome 4 |
| Location: | 2391681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221301 | CYCD5 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGGGGTTGTCCGGTGGAGCCGCAACAGGCTACGACAGGCCAGCCAG |
| Internal bar code: | CTCAGGTTTGGTATCGCTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4071 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGCACCTACTACAGCC |
| Suggested primer 2: | ACGGGACCTGCACAAACTAG |