| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035678 |
| Chromosome: | chromosome 7 |
| Location: | 2459575 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329200 | SPL21,SPLH1 | (1 of 3) PTHR18934:SF143 - DEAD/DEAH BOX HELICASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGGCATATGCAATGTAGTTGACTGTGTTGATTCGCAGCTGCAGCCT |
| Internal bar code: | GCATCTGAGCGGGTCCTCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2350 |
| LEAP-Seq percent confirming: | 36.3636 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACACGCGAAAGGCTATG |
| Suggested primer 2: | GCATGTGACATCTGGACCGA |