| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035679 |
| Chromosome: | chromosome 14 |
| Location: | 2968076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627500 | (1 of 1) PTHR18937//PTHR18937:SF259 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACATGCCGCCGGCGGCGGCGGCGGCGGCGGCGGCGACGGCTGGAGCGG |
| Internal bar code: | TTATGTTTTGTTTGGCTAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1144 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCGCGTGTGTGGATAC |
| Suggested primer 2: | GTAAAAGGGGGAGTGGGAGC |