| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035698 |
| Chromosome: | chromosome 16 |
| Location: | 649844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691750 | AMI1 | putative formamidase/acetamidase; (1 of 1) 3.5.1.49 - Formamidase / Formamide amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCTTGCCCGGGGTGCGCCAGGCAGCATACCGCGTCGGCCCACACCA |
| Internal bar code: | TTGAGGAACTGCCTGGTTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3147 |
| LEAP-Seq percent confirming: | 65.1163 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTGTGTGTGGA |
| Suggested primer 2: | CGTCTACAGCATCCCCATCC |