Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.035703 |
Chromosome: | chromosome 7 |
Location: | 1797202 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g325727 | (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCGGCCGCCAGAACATCCACCACCGCTGGCGCTGGGGGCGCCTTGCT |
Internal bar code: | GGCTGGGTAAGATAATGCTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1520 |
LEAP-Seq percent confirming: | 95.4545 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACATGCATCAACACACGC |
Suggested primer 2: | TTGTCCAGGTCAATGCCCTC |