Insertion junction: CLIP2.035703_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035703
Chromosome:chromosome 7
Location:1797208
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325727 (1 of 3) PTHR10566:SF76 - PROTEIN Y32H12A.7 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTGGGCGGGTGCTGCACGGCGCACTGTCACTGGCGCGCGCGGCCTCA
Internal bar code:CGGGGTTAGCACTGAGGGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1923
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTCCAGGTCAATGCCCTC
Suggested primer 2:ACACATGCATCAACACACGC