| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.035705 |
| Chromosome: | chromosome 2 |
| Location: | 6964725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389282 | PF6-IP3,C1a-32,FAP114 | (1 of 2) PF14769 - Flagellar C1a complex subunit C1a-32 (CLAMP); Coiled-Coil Flagellar Associated Protein 114 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAAAAGCGCCCACCCCATCACGTCTGGGGTGCATTACTTCCTCTGTA |
| Internal bar code: | GGTTTTGCGATATACGATGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2336 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGCTTCCCTCTTCCAG |
| Suggested primer 2: | ACATTGGGTTGGCGTGATCT |