Insertion junction: CLIP2.035709_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035709
Chromosome:chromosome 7
Location:3532632
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGAAGGTCAAATCGTAGTGGGTCTGTCGTCATACAGCTGCTGCGTGGC
Internal bar code:ATCATCAACCATAGGCCTACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1713
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCCCACGTGTTTGTTT
Suggested primer 2:CAGGTGTGAAGTACGGCAGT