| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.035724 |
| Chromosome: | chromosome 2 |
| Location: | 4647001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACTTTGACACAGTTACGACCGCGCAGGTGCGCTATGCGACCCCCCG |
| Internal bar code: | ATCACGATTTCGCTTTCCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2047 |
| LEAP-Seq percent confirming: | 76.1905 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGTTCTCGCGCTTTCG |
| Suggested primer 2: | TCCTTCTTGCAGCACTCCAG |