| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.035727 |
| Chromosome: | chromosome 2 |
| Location: | 3768221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100800 | (1 of 1) PTHR22847//PTHR22847:SF410 - WD40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 27 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACAGTTGCAGGCACGCGGGGGAGCGCCGCACCGCCCCGGATGCAC |
| Internal bar code: | TTTGCCAACGCATATCGTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGTCATGGCCTCATTG |
| Suggested primer 2: | TAGCTCGTTGGAATGTGCGT |