| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035731 |
| Chromosome: | chromosome 12 |
| Location: | 1997061 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510900 | HSLU1,HUV1,CLPY1 | ATP-dependent subunit of the HslUV protease; (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCGGGCAGCAAGCGGCGCAGCCGCCGATCGCGGTCGGGCAACAGCCCG |
| Internal bar code: | TCTATGGTCAAGTAGTCTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTGCACCTGTGACCTA |
| Suggested primer 2: | GGGGTTTAGGGAAGGGAAGC |