| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.035765 |
| Chromosome: | chromosome 3 |
| Location: | 6677977 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195388 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGTGCCAGGCAAGGTCCTTTCCGTGTCGACAGCGAGGATCCCCGG |
| Internal bar code: | GCGTACGGTTCACCTGTATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1942 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCGTATTCGCGTGAGAG |
| Suggested primer 2: | AAACCCAACATCAACAGCGC |