| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035775 |
| Chromosome: | chromosome 13 |
| Location: | 3925573 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590010 | (1 of 8) 3.1.1.13//3.1.1.3 - Sterol esterase / Triterpenol esterase // Triacylglycerol lipase / Triglyceride lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGGCGAGGCTGGCTGCGCGGGTGCACGGACTCGTGTCGCACTCGAAC |
| Internal bar code: | ATAACGTGGTCTTGATAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4213 |
| LEAP-Seq percent confirming: | 24.1379 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTATCCAAGCGCTCGCAG |
| Suggested primer 2: | GAACCACGTCAGGCTGTACA |