| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035776 |
| Chromosome: | chromosome 12 |
| Location: | 2723160 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504750 | HTA16,HTA43 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACAAGAAGAACCGCATCGTGCCCCGCCACATCCAGCTGGCCATCCGT |
| Internal bar code: | CTGTAGCCCCCGGCGAAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3418 |
| LEAP-Seq percent confirming: | 84.3373 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCCAACACTCAGCAGC |
| Suggested primer 2: | GAGCGGTTGTTTGACCCAAC |