Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.035782 |
Chromosome: | chromosome 13 |
Location: | 5025813 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g606300 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGTCCAGACCCTAGGGGCTATGCGGTGCTCTACTAGGATGCACTTGG |
Internal bar code: | ATAATGGAAAAATTACGCGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2553 |
LEAP-Seq percent confirming: | 43.9024 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCAAAGGGTGGGGTTTCAA |
Suggested primer 2: | CACTTTGAAACATGGCCGCA |