| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035808 |
| Chromosome: | chromosome 16 |
| Location: | 4445855 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668050 | (1 of 97) PF13650 - Aspartyl protease (Asp_protease_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGCGGGCTGCGCGGTGTCTTGCGCAACGGGAATGATACCACCTACGA |
| Internal bar code: | GTTCGCAATAACTCGAACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1987 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGGCGGTTGCTCATTCT |
| Suggested primer 2: | AACACACACAATGAAGCGCC |