| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035811 |
| Chromosome: | chromosome 5 |
| Location: | 2490198 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239100 | FPG2 | (1 of 1) 6.3.2.17 - Tetrahydrofolate synthase / Tetrahydrofolylpolyglutamate synthase; Putative folylpolyglutamate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCATACTAAGTATTGTGTGCTGTGTTACGCAAAATTAATCAAATACAC |
| Internal bar code: | GATCTGCGTCATTTTGAGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGAGGTACCGGTAGTCA |
| Suggested primer 2: | TTAGGGAAACAGGCGTGGAC |