| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035824 |
| Chromosome: | chromosome 17 |
| Location: | 475748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699000 | PAT1 | Phosphate acetyltransferase; (1 of 2) 2.3.1.8 - Phosphate acetyltransferase / Phosphotransacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACGCACCCGGTCGGGCAGGCACATGAAGAAGATGGAGGACACGAGGC |
| Internal bar code: | TGTTTTAATACTATTGGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1498 |
| LEAP-Seq percent confirming: | 91.4894 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACACACACGAACACCAC |
| Suggested primer 2: | ATCCCAGGGTGGAGTAGGAC |