Insertion junction: CLIP2.035837_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035837
Chromosome:chromosome 6
Location:8603205
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g309800 MOT57,MOT2 Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGATCGCACACTCTGCCATGCGTCAGGCCGTTTATGAATGCGCTGCG
Internal bar code:TTCTGAGGTCTATTTCAACTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:4945
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:TATTCGTCGCGCTCCTGTAC
Suggested primer 2:CCCTACCCCTTTCCGTCAAC