Insertion junction: CLIP2.035837_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035837
Chromosome:chromosome 6
Location:8603211
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g309800 MOT57,MOT2 Predicted protein in motile organisms; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGGTGCCAACCGCCCGTCCGCCCATCCATTCCATATACGCACACAAGC
Internal bar code:TTCTGAGGTCTATTTCAACTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1733
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTACCCCTTTCCGTCAAC
Suggested primer 2:TATTCGTCGCGCTCCTGTAC