| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.035860 |
| Chromosome: | chromosome 10 |
| Location: | 4102018 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448350 | (1 of 3) PTHR10361 - SODIUM-BILE ACID COTRANSPORTER | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCAGCTTGACGCGAACTTGACGCTGGGCCTGGCCTGCTTCTTCCTGG |
| Internal bar code: | CCTTTGTGATCATGGGCGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1727 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGTGGTGGAAGGCGTAGG |
| Suggested primer 2: | TTCTTAGGTTTCCGCGTGCT |