| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.035871 |
| Chromosome: | chromosome 1 |
| Location: | 7037315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050350 | RBL6 | Rhomboid-like protease; (1 of 1) PTHR12917//PTHR12917:SF19 - ASPARTYL PROTEASE DDI-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCACGAAGGCCAGCATGTTGAAGGTCACGTGCAGCAGGCCCCCGTGC |
| Internal bar code: | GCCGACTGTCTTTGGGTCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3650 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTCCTCATCAGCGGCAT |
| Suggested primer 2: | CACGTACGTAATGCGCAAGG |