Insertion junction: CLIP2.035872_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.035872
Chromosome:chromosome 11
Location:3244718
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g476250 (1 of 37) PF13639 - Ring finger domain (zf-RING_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTGCCACCAGTCCACGACTCGCCACCCACTCTGCCCCTAAACGTTTC
Internal bar code:ACGTGCGTTGAAGCCAGGATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCCGGTTCGACGTCAG
Suggested primer 2:ATCATCGACGGCTTCCACTC