| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.035873 |
| Chromosome: | chromosome 6 |
| Location: | 4920664 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280950 | PYK2 | Pyruvate kinase 2; (1 of 6) 2.7.1.40 - Pyruvate kinase / Phosphoenolpyruvate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAATGCAATAGAGCGAGGAGGAGAGCGTGGCCTATGCTTGACAACGGAGT |
| Internal bar code: | ACGCATTGGGCTTTTGACTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1258 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTTGGAGATGTAGGGC |
| Suggested primer 2: | ACGAACACAGCAAATTGCCC |