| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035873 |
| Chromosome: | chromosome 6 |
| Location: | 4920669 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280950 | PYK2 | Pyruvate kinase 2; (1 of 6) 2.7.1.40 - Pyruvate kinase / Phosphoenolpyruvate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTAATTCGTGCTGGTATCTACAACCCCCGCCTCGCTCCCGCAGCTTC |
| Internal bar code: | ACGCATTGGGCTTTTGACTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1885 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAACACAGCAAATTGCCC |
| Suggested primer 2: | CCAGCTTGGAGATGTAGGGC |