Insertion junction: CLIP2.035875_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.035875
Chromosome:chromosome 8
Location:1394550
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g364250 (1 of 1) PF09174 - Maf1 regulator (Maf1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGATCCTGACCATGAACCATATCTACCCGGACCACGACTTCAGCCTCC
Internal bar code:TTAGACACACACTAAACTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1157
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGAGGCCACCATCTCAT
Suggested primer 2:TTCGTGTGCTACTCGCCTTT