| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035875 |
| Chromosome: | chromosome 12 |
| Location: | 9645321 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801499 | 3'UTR | ||
| Cre35.g759447 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGGCATGCACGTCGGTTACTCCAAGCATTCAAGAGCGAGCGCACTA |
| Internal bar code: | GTCAATCTGACCGCTGTGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 80.4878 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGAGACGTACAGGGGA |
| Suggested primer 2: | CTGTTGCTGTTGCTGTTGCT |