| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.035880 |
| Chromosome: | chromosome 2 |
| Location: | 5428033 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAGCCTCAGCCGAGCGAGGTGAGGCGAGGTAACCCGAGGCCATGCCC |
| Internal bar code: | TAAATGGAGCACGCTGAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1330 |
| LEAP-Seq percent confirming: | 98.4375 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTACACTCCCAGAACC |
| Suggested primer 2: | ATGGCGCAGGATTGTGGTAA |