Insertion junction: CLIP2.035892_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.035892
Chromosome:chromosome 17
Location:261435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697650 (1 of 1) K01354 - oligopeptidase B (ptrB) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACGTGGCTGGGTGGCTGCGCTCCACACGTACACACAGACCTCGCCA
Internal bar code:CATTACAGCTTGTTTCCTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGGAACTGGCTCACACC
Suggested primer 2:CGTATGCGTGCGTGTGTTAG