Insertion junction: CLIP2.035908_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.035908
Chromosome:chromosome 7
Location:3416197
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g335300 KAS2 (1 of 1) 2.3.1.179 - Beta-ketoacyl-[acyl-carrier-protein] synthase II / KASII; 3-ketoacyl-ACP-synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTGTGCTTCTGCGCTGCATCTTACTGCATCTTACTGCGCTGCACTGC
Internal bar code:GTTATGTATGTTGTCAATAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:4309
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCATACTCCTCCAGCACC
Suggested primer 2:TACGGTGAATATGTCCGCGG