Insertion junction: CLIP2.035922_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.035922
Chromosome:chromosome 9
Location:2173889
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g395550 (1 of 1) PF05991 - YacP-like NYN domain (NYN_YacP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTCCTCTTGTTCTCTCTGACACACACACGCACGCACGGACAGCTGGG
Internal bar code:CGCTGGTTTGGTTTACTTATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:339
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTCTTCCCCTTCATCGC
Suggested primer 2:CCCCACTCCTCAATGCACAT